Excessive-level Multiplexing in Digital PCR With Intercalating Dyes by Coupling Actual-Time Kinetics and Melting Curve Evaluation
Digital polymerase chain response (dPCR) is a mature approach that has enabled scientific breakthroughs in a number of fields. Nonetheless, this know-how is primarily utilized in analysis environments with high-level multiplexing representing a serious problem. Right here, we suggest a novel technique for multiplexing, known as amplification and melting curve evaluation (AMCA), which leverages the kinetic data in real-time amplification information and the thermodynamic melting profile utilizing an inexpensive intercalating dye (EvaGreen).
The strategy trains a system comprised of supervised machine studying fashions for correct classification, by advantage of the massive quantity of information from dPCR platforms. As a case research, we develop a brand new 9-plex assay to detect mobilised colistin resistant ( mcr ) genes as clinically related targets for antimicrobial resistance. Over 100,000 amplification occasions have been analysed, and for the optimistic reactions, the AMCA method experiences a classification accuracy of 99.33 ± 0.13%, a rise of 10.0% over utilizing melting curve evaluation. This work supplies an inexpensive technique of high-level multiplexing with out fluorescent probes, extending the advantages of dPCR in analysis and scientific settings.
ayurvedandindia
GPR110 antibody
70R-31396
Fitzgerald
100 ug
EUR 392.4
Description: Rabbit polyclonal GPR110 antibody
GPR110 antibody
70R-51217
Fitzgerald
100 ul
EUR 292.8
Description: Purified Polyclonal GPR110 antibody
GPR110 Antibody
1-CSB-PA002753
Cusabio
Description: A polyclonal antibody against GPR110. Recognizes GPR110 from Human. This antibody is Unconjugated. Tested in the following application: WB, IF, ELISA;WB:1/500-1/2000.IF:1/200-1/1000.ELISA:1/40000
Polyclonal GPR110 Antibody
APR12205G
Leading Biology
0.1ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR110 . This antibody is tested and proven to work in the following applications:
GPR110 Conjugated Antibody
C44961
SAB
100ul
EUR 476.4
GPR110 Polyclonal Antibody
ABP51449-003ml
Abbkine
0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of GPR110 from Human. This GPR110 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human GPR110 at AA range: 810-890
GPR110 Polyclonal Antibody
ABP51449-01ml
Abbkine
0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of GPR110 from Human. This GPR110 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human GPR110 at AA range: 810-890
GPR110 Polyclonal Antibody
ABP51449-02ml
Abbkine
0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of GPR110 from Human. This GPR110 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the C-terminal region of human GPR110 at AA range: 810-890
GPR110 Polyclonal Antibody
ES2448-100ul
ELK Biotech
100ul
EUR 334.8
Description: A Rabbit Polyclonal antibody against GPR110 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA
GPR110 Polyclonal Antibody
ES2448-50ul
ELK Biotech
50ul
EUR 248.4
Description: A Rabbit Polyclonal antibody against GPR110 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA
anti-GPCR GPR110
YF-PA22867
Abfrontier
50 ul
EUR 435.6
Description: Mouse polyclonal to GPCR GPR110
anti-GPCR GPR110
YF-PA22868
Abfrontier
50 ug
EUR 435.6
Description: Mouse polyclonal to GPCR GPR110
G Protein-Coupled Receptor 110 (GPR110) Antibody
20-abx147426
Abbexa
G Protein-Coupled Receptor 110 (GPR110) Antibody
20-abx007923
Abbexa
EUR 360.00
EUR 526.80
EUR 226.80
G Protein-Coupled Receptor 110 (GPR110) Antibody
20-abx015251
Abbexa
EUR 376.80
EUR 117.60
EUR 477.60
EUR 594.00
100 ug
10 ug
200 ug
300 µg
GPR110 siRNA
20-abx918404
Abbexa
GPR110 siRNA
20-abx918405
Abbexa
GPR110 Blocking Peptide
20-abx064092
Abbexa
GPR110 Blocking Peptide
DF4891-BP
Affbiotech
1mg
EUR 234
GPR110 Blocking Peptide
DF2796-BP
Affbiotech
1mg
EUR 234
Mouse GPR110 shRNA Plasmid
20-abx978802
Abbexa
Human GPR110 shRNA Plasmid
20-abx966836
Abbexa
anti- GPR105 antibody
FNab03588
FN Test
100µg
EUR 658.5
Description: Antibody raised against GPR105
anti- GPR107 antibody
FNab03589
FN Test
100µg
EUR 702
Description: Antibody raised against GPR107
anti- GPR108 antibody
FNab03590
FN Test
100µg
EUR 658.5
Description: Antibody raised against GPR108
anti- GPR116 antibody
FNab03591
FN Test
100µg
EUR 658.5
Description: Antibody raised against GPR116
anti- GPR132 antibody
FNab03593
FN Test
100µg
EUR 606.3
Description: Antibody raised against GPR132
anti- GPR137 antibody
FNab03594
FN Test
100µg
EUR 606.3
Description: Antibody raised against GPR137
anti- GPR142 antibody
FNab03597
FN Test
100µg
EUR 702
Description: Antibody raised against GPR142
anti- GPR161 antibody
FNab03598
FN Test
100µg
EUR 606.3
Description: Antibody raised against GPR161
anti- GPR162 antibody
FNab03599
FN Test
100µg
EUR 702
Description: Antibody raised against GPR162
anti- GPR177 antibody
FNab03601
FN Test
100µg
EUR 658.5
Description: Antibody raised against GPR177
anti- GPR137B antibody
FNab03595
FN Test
100µg
EUR 702
Description: Antibody raised against GPR137B
anti- GPR17 antibody
FNab03600
FN Test
100µg
EUR 658.5
Description: Antibody raised against GPR17
anti- GPR22 antibody
FNab03603
FN Test
100µg
EUR 606.3
Description: Antibody raised against GPR22
anti- GPR35 antibody
FNab03605
FN Test
100µg
EUR 702
Description: Antibody raised against GPR35
anti- GPR50 antibody
FNab03607
FN Test
100µg
EUR 658.5
Description: Antibody raised against GPR50
anti- GPR50 antibody
FNab03608
FN Test
100µg
EUR 658.5
Description: Antibody raised against GPR50
anti- GPR81 antibody
FNab03610
FN Test
100µg
EUR 702
Description: Antibody raised against GPR81
anti- GPR83 antibody
FNab03611
FN Test
100µg
EUR 702
Description: Antibody raised against GPR83
GPR110 ORF Vector (Human) (pORF)
ORF020338
ABM
1.0 ug DNA
EUR 486
Gpr110 ORF Vector (Mouse) (pORF)
ORF046467
ABM
1.0 ug DNA
EUR 607.2
anti- GPR183/EBI2 antibody
FNab03602
FN Test
100µg
EUR 658.5
Description: Antibody raised against GPR183/EBI2
Anti-OR51E1/Gpr164 Antibody
A30874
BosterBio
100ul
EUR 476.4
Description: Rabbit Polyclonal OR51E1/Gpr164 Antibody. Validated in IF, WB and tested in Human.
anti- GPR3 antibody
FNab03604
FN Test
100µg
EUR 702
Description: Antibody raised against GPR3
anti- GPR101-Specific antibody
FNab03587
FN Test
100µg
EUR 702
Description: Antibody raised against GPR101-Specific
anti- GPR123-Specific antibody
FNab03592
FN Test
100µg
EUR 702
Description: Antibody raised against GPR123-Specific
anti- GPR139-Specific antibody
FNab03596
FN Test
100µg
EUR 606.3
Description: Antibody raised against GPR139-Specific
Anti-FFAR3/Gpr41 Antibody
A02437
BosterBio
100ul
EUR 476.4
Description: Rabbit Polyclonal FFAR3/Gpr41 Antibody. Validated in IF and tested in Human, Mouse.
anti- GPR58/TAAR2 antibody
FNab03609
FN Test
100µg
EUR 658.5
Description: Antibody raised against GPR58/TAAR2
Anti-SUCNR1/Gpr91 Antibody
A08485
BosterBio
100ul
EUR 476.4
Description: Rabbit Polyclonal SUCNR1/Gpr91 Antibody. Validated in WB and tested in Human, Mouse, Rat.
anti- GPR37/Pael-R antibody
FNab03606
FN Test
100µg
EUR 606.3
Description: Antibody raised against GPR37/Pael-R
GPR110 sgRNA CRISPR Lentivector set (Human)
K0894901
ABM
3 x 1.0 ug
EUR 406.8
Gpr110 sgRNA CRISPR Lentivector set (Mouse)
K3842201
ABM
3 x 1.0 ug
EUR 406.8
Anti-GPCR GPR40/FFAR1 Antibody
A01349-2
BosterBio
100ug/vial
EUR 352.8
Gpr110 3'UTR GFP Stable Cell Line
TU158961
ABM
1.0 ml
Ask for price
GPR110 3'UTR GFP Stable Cell Line
TU059201
ABM
1.0 ml
EUR 1825.2
Anti-GPR110 Antibody
A30791
BosterBio
100ul
EUR 476.4
Description: Rabbit Polyclonal GPR110 Antibody. Validated in IF, WB and tested in Human.
Anti-GPR110 antibody
STJ93320
St John's Laboratory
200 µl
EUR 236.4
Description: Rabbit polyclonal to GPR110.
GPR110 Protein Vector (Mouse) (pPM-C-HA)
PV185868
ABM
500 ng
EUR 1278
GPR110 Protein Vector (Human) (pPM-C-HA)
PV081351
ABM
500 ng
EUR 662.4
GPR110 Protein Vector (Mouse) (pPB-C-His)
PV185866
ABM
500 ng
EUR 1278
GPR110 Protein Vector (Mouse) (pPB-N-His)
PV185867
ABM
500 ng
EUR 1278
GPR110 Protein Vector (Mouse) (pPM-C-His)
PV185869
ABM
500 ng
EUR 1278
GPR110 Protein Vector (Human) (pPB-C-His)
PV081349
ABM
500 ng
EUR 662.4
GPR110 Protein Vector (Human) (pPB-N-His)
PV081350
ABM
500 ng
EUR 662.4
GPR110 Protein Vector (Human) (pPM-C-His)
PV081352
ABM
500 ng
EUR 662.4
GPR110 3'UTR Luciferase Stable Cell Line
TU009201
ABM
1.0 ml
EUR 1825.2
Gpr110 3'UTR Luciferase Stable Cell Line
TU108961
ABM
1.0 ml
Ask for price
Anti-LGR4/Gpr48 Monoclonal Antibody
M02134
BosterBio
100uL
EUR 531.6
Description: Mouse Monoclonal LGR4/Gpr48 Antibody. Validated in IHC, WB and tested in Human.
GPR110 sgRNA CRISPR Lentivector (Human) (Target 1)
K0894902
ABM
1.0 ug DNA
EUR 184.8
GPR110 sgRNA CRISPR Lentivector (Human) (Target 2)
K0894903
ABM
1.0 ug DNA
EUR 184.8
GPR110 sgRNA CRISPR Lentivector (Human) (Target 3)
K0894904
ABM
1.0 ug DNA
EUR 184.8
Gpr110 sgRNA CRISPR Lentivector (Mouse) (Target 1)
K3842202
ABM
1.0 ug DNA
EUR 184.8
Gpr110 sgRNA CRISPR Lentivector (Mouse) (Target 2)
K3842203
ABM
1.0 ug DNA
EUR 184.8
Gpr110 sgRNA CRISPR Lentivector (Mouse) (Target 3)
K3842204
ABM
1.0 ug DNA
EUR 184.8
Polyclonal Goat Anti-TAS1R2 / GPR71 Antibody
AMM05957G
Leading Biology
0.1 mg
EUR 580.8
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Goat Anti-TAS1R2 / GPR71 . This antibody is tested and proven to work in the following applications:
Anti Human GPR120 Monoclonal Antibody (Clone No. 2B6)
KG138
Sceti
each
EUR 856.8
Description: The Anti Human GPR120 Monoclonal Antibody (Clone No. 2B6) is available in Europe and for worldwide shipping via Gentaur.
Anti-LGR5/GPR49 Rabbit Monoclonal Antibody
M00239
BosterBio
100ug/vial
EUR 476.4
Description: Rabbit Monoclonal LGR5/GPR49 Antibody. Validated in Flow Cytometry, IP, WB and tested in Human, Mouse, Rat.
Anti-GPCR GPR7/NPBWR1 Antibody
A08247-1
BosterBio
100ug/vial
EUR 352.8
Anti Mouse GPR40 Monoclonal Antibody (Clone No. G16)
KG116
Sceti
each
EUR 919.2
Description: The Anti Mouse GPR40 Monoclonal Antibody (Clone No. G16) is available in Europe and for worldwide shipping via Gentaur.
Mouse G- protein coupled receptor 110, Gpr110 ELISA KIT
ELI-09748m
Lifescience Market
96 Tests
EUR 1038
Rabbit Anti Human Gpr15 (N-Terminal) Polyclonal Antibody
CPBT-65692RH
Creative Diagnostics
0.1 mg
EUR 696
GPR105 (GPR105) Antibody
abx233588-100ug
Abbexa
100 ug
EUR 610.8
GPR105 (GPR105) Antibody
20-abx217588
Abbexa
GPR105 (GPR105) Antibody
20-abx008376
Abbexa
EUR 360.00
EUR 526.80
EUR 226.80
GPR80 / GPR99 Antibody
20-abx147443
Abbexa
GPR87 / GPR95 Antibody
20-abx147553
Abbexa
GPR92 / GPR93 Antibody
20-abx147570
Abbexa
GPR86 / GPR94 Antibody
20-abx147612
Abbexa
GPR80/GPR99 Antibody
44941-100ul
SAB
100ul
EUR 302.4
GPR80/GPR99 Antibody
44941-50ul
SAB
50ul
EUR 224.4
GPR83/GPR72 Antibody
44944-100ul
SAB
100ul
EUR 302.4
GPR83/GPR72 Antibody
44944-50ul
SAB
50ul
EUR 224.4
GPR86/GPR94 Antibody
44945-100ul
SAB
100ul
EUR 302.4
GPR86/GPR94 Antibody
44945-50ul
SAB
50ul
EUR 224.4
GPR87/GPR95 Antibody
44946-100ul
SAB
100ul
EUR 302.4
GPR87/GPR95 Antibody
44946-50ul
SAB
50ul
EUR 224.4
GPR92/GPR93 Antibody
44948-100ul
SAB
100ul
EUR 302.4
GPR92/GPR93 Antibody
44948-50ul
SAB
50ul
EUR 224.4
GPR83/GPR72 Antibody
DF4977
Affbiotech
200ul
EUR 420
GPR87/GPR95 Antibody
DF4978
Affbiotech
200ul
EUR 420
GPR92/GPR93 Antibody
DF4979
Affbiotech
200ul
EUR 420
GPR86/GPR94 Antibody
DF5139
Affbiotech
200ul
EUR 420
GPR80/GPR99 Antibody
DF2765
Affbiotech
200ul
EUR 420
GPR83/GPR72 Antibody
DF2768
Affbiotech
200ul
EUR 420
GPR86/GPR94 Antibody
DF2770
Affbiotech
200ul
EUR 420
GPR87/GPR95 Antibody
DF2771
Affbiotech
200ul
EUR 420
GPR92/GPR93 Antibody
DF2774
Affbiotech
200ul
EUR 420
GPR109 Antibody
abx217169-100ug
Abbexa
100 ug
EUR 526.8
GPR182 antibody
20R-AR029
Fitzgerald
50 ug
EUR 787.2
Description: Rabbit polyclonal GPR182 antibody
GPR182 antibody
20R-AR030
Fitzgerald
50 ug
EUR 787.2
Description: Rabbit polyclonal GPR182 antibody
GPR182 antibody
20R-AR031
Fitzgerald
50 ug
EUR 787.2
Description: Rabbit polyclonal GPR182 antibody
GPR182 antibody
20R-AR032
Fitzgerald
50 ug
EUR 787.2
Description: Rabbit polyclonal GPR182 antibody
GPR182 antibody
20R-AR033
Fitzgerald
50 ug
EUR 787.2
Description: Rabbit polyclonal GPR182 antibody
GPR171 antibody
20R-GR028
Fitzgerald
50 ug
EUR 787.2
Description: Rabbit polyclonal GPR171 antibody
GPR175 antibody
20R-GR031
Fitzgerald
50 ug
EUR 787.2
Description: Rabbit polyclonal GPR175 antibody
GPR124 antibody
20R-GR039
Fitzgerald
50 ug
EUR 787.2
Description: Rabbit polyclonal GPR124 antibody
GPR115 antibody
20R-GR040
Fitzgerald
50 ug
EUR 787.2
Description: Rabbit polyclonal GPR115 antibody
GPR153 antibody
20R-GR042
Fitzgerald
50 ug
EUR 787.2
Description: Rabbit polyclonal GPR153 antibody
GPR132 antibody
20R-GR045
Fitzgerald
50 ug
EUR 787.2
Description: Rabbit polyclonal GPR132 antibody
GPR174 antibody
20R-GR051
Fitzgerald
50 ug
EUR 787.2
Description: Rabbit polyclonal GPR174 antibody
GPR152 antibody
20R-GR058
Fitzgerald
50 ug
EUR 787.2
Description: Rabbit polyclonal GPR152 antibody
GPR151 antibody
20R-GR062
Fitzgerald
50 ug
EUR 787.2
Description: Rabbit polyclonal GPR151 antibody
GPR149 antibody
20R-GR065
Fitzgerald
50 ug
EUR 787.2
Description: Rabbit polyclonal GPR149 antibody
GPR149 antibody
20R-GR066
Fitzgerald
50 ug
EUR 787.2
Description: Rabbit polyclonal GPR149 antibody
GPR160 antibody
20R-GR067
Fitzgerald
50 ug
EUR 787.2
Description: Rabbit polyclonal GPR160 antibody
GPR114 antibody
23178-100ul
SAB
100ul
EUR 468
GPR182 Antibody
37423-100ul
SAB
100ul
EUR 302.4
GPR124 Antibody
37603-100ul
SAB
100ul
EUR 302.4
GPR171 Antibody
37604-100ul
SAB
100ul
EUR 302.4
GPR143 Antibody
39269-100ul
SAB
100ul
EUR 468
GPR139 Antibody
44331-100ul
SAB
100ul
EUR 302.4
GPR139 Antibody
44331-50ul
SAB
50ul
EUR 224.4
GPR112 Antibody
44576-100ul
SAB
100ul
EUR 302.4
GPR112 Antibody
44576-50ul
SAB
50ul
EUR 224.4
GPR125 Antibody
44577-100ul
SAB
100ul
EUR 302.4
GPR125 Antibody
44577-50ul
SAB
50ul
EUR 224.4
GPR179 Antibody
44578-100ul
SAB
100ul
EUR 302.4
GPR179 Antibody
44578-50ul
SAB
50ul
EUR 224.4
GPR128 Antibody
44911-100ul
SAB
100ul
EUR 302.4
GPR128 Antibody
44911-50ul
SAB
50ul
EUR 224.4
GPR133 Antibody
44912-100ul
SAB
100ul
EUR 302.4
GPR133 Antibody
44912-50ul
SAB
50ul
EUR 224.4
GPR136 Antibody
44913-100ul
SAB
100ul
EUR 302.4
GPR136 Antibody
44913-50ul
SAB
50ul
EUR 224.4
GPR148 Antibody
44914-100ul
SAB
100ul
EUR 302.4
GPR148 Antibody
44914-50ul
SAB
50ul
EUR 224.4
GPR141 Antibody
44962-100ul
SAB
100ul
EUR 302.4
GPR141 Antibody
44962-50ul
SAB
50ul
EUR 224.4
GPR161 Antibody
44963-100ul
SAB
100ul
EUR 302.4
GPR161 Antibody
44963-50ul
SAB
50ul
EUR 224.4
GPR162 Antibody
44964-100ul
SAB
100ul
EUR 302.4
GPR162 Antibody
44964-50ul
SAB
50ul
EUR 224.4
GPR176 Antibody
44965-100ul
SAB
100ul
EUR 302.4
GPR176 Antibody
44965-50ul
SAB
50ul
EUR 224.4
GPR101 antibody
70R-30903
Fitzgerald
100 ug
EUR 392.4
Description: Rabbit polyclonal GPR101 antibody
GPR107 antibody
70R-30904
Fitzgerald
100 ug
EUR 392.4
Description: Rabbit polyclonal GPR107 antibody
GPR108 antibody
70R-30905
Fitzgerald
100 ug
EUR 392.4
Description: Rabbit polyclonal GPR108 antibody
GPR113 antibody
70R-30906
Fitzgerald
100 ug
EUR 392.4
Description: Rabbit polyclonal GPR113 antibody
GPR114 antibody
70R-30907
Fitzgerald
100 ug
EUR 392.4
Description: Rabbit polyclonal GPR114 antibody
GPR115 antibody
70R-30908
Fitzgerald
100 ug
EUR 392.4
Description: Rabbit polyclonal GPR115 antibody
GPR116 antibody
70R-30909
Fitzgerald
100 ug
EUR 392.4
Description: Rabbit polyclonal GPR116 antibody
GPR123 antibody
70R-30910
Fitzgerald
100 ug
EUR 392.4
Description: Rabbit polyclonal GPR123 antibody
GPR124 antibody
70R-30911
Fitzgerald
100 ug
EUR 392.4
Description: Rabbit polyclonal GPR124 antibody
GPR126 antibody
70R-30912
Fitzgerald
100 ug
EUR 392.4
Description: Rabbit polyclonal GPR126 antibody
GPR132 antibody
70R-30913
Fitzgerald
100 ug
EUR 392.4
Description: Rabbit polyclonal GPR132 antibody
GPR133 antibody
70R-30914
Fitzgerald
100 ug
EUR 392.4
Description: Rabbit polyclonal GPR133 antibody
GPR135 antibody
70R-30915
Fitzgerald
100 ug
EUR 392.4
Description: Rabbit polyclonal GPR135 antibody
Detection of Helicobacter pylori in gastric most cancers tissue via histopathology, immunohistochemistry and real-time reverse transcription-PCR
Intention: Helicobacter pylori is often detected primarily based on hematoxylin-eosin (H-E) options, however, immunohistochemistry (IHC) and real-time PCR (RT-PCR) are extra exact in chronic-gastritis. We evaluated the relevance of those checks in Peruvian gastric most cancers samples.
Supplies & strategies: We carried out and evaluated H-E, IHC staining and RT-PCR in 288 gastric tumors. Slides have been independently evaluated by three pathologists.
Outcomes: H. pylori was detected in 167/287 via H-E, 140/288 via IHC and 175/288 via RT-PCR, and positive-status have been related (p < 0.001). H. pylori detection by H-E had an excellent concordance with IHC (kappa index = 0.632) however poor with RT-PCR (kappa index = 0.317). Greater median gene-copies have been present in excessive H. pylori density via H-E or IHC (p < 0.001).
Conclusion : H-E analysis is correct in gastric most cancers, and IHC and RT-PCR can complement its outcomes.
Analytical validation of the droplet digital PCR assay for prognosis of spinal muscular atrophy
Background: Spinal muscular atrophy (SMA) is a progressive motor neuron illness attributable to homozygote lack of exon 7 on the survival motor neuron 1 (SMN1) gene. The severity of the SMA phenotype is influenced by copies of SMN2, a gene that’s extremely homologous with SMN1.
Strategies: We validated analytical efficiency of droplet digital polymerase chain response (ddPCR) for detection of copy quantity variation of SMN1 and SMN2 genes for prognosis of SMA utilizing scientific samples. For accuracy efficiency analysis, ddPCR outcomes have been in contrast with these of multiplex ligation-dependent probe amplification (MLPA) as a reference normal. Copy numbers of SMN1/SMN2 exon 7 from 200 scientific samples have been concordant between ddPCR and MLPA.
Outcomes: For all samples, the copy variety of SMN1/SMN2 exon 7 was concordant between MLPA and ddPCR. The ddPCR additionally confirmed acceptable levels of repeatability and whole imprecision.
Conclusion: Due to this fact, ddPCR is predicted to be helpful for SMA prognosis and to foretell phenotypic severity of SMA sufferers by figuring out the copy quantity ofSMN2in scientific laboratories.
A multiplex PCR equipment for the detection of three main virulent genes in Enterococcus faecalis
A multiplex PCR equipment that detects three main virulence genes, gelE, hyl and asaI, in Enterococcus faecalis was developed. Analyses of the accessible sequences of the three main virulence genes and the designed primers allowed us to develop the three-gene , multiplex PCR protocol that maintained the specificity of every primer pair. The ensuing three amplicon bands for gelE, hyl and asaI have been even and distinct with product sizes of 213, 273 and 713 bp, respectively.
The multiplex PCR process was validated with a complete of 243 E. faecalis strains that included 02 ATCC strains, 109 isolates from marine samples (sediment, water and sea meals), 22 isolates from cattle fodder, 79 isolates contemporary water samples and 31isolates from nosocomial samples. Specificity of the equipment was indicated by amplification of solely three main virulence genes gelE, hyl and asaI, and with none nonspecific bands . Exams for the restrict of detection revealed that amplified genes from the pattern with a minimal of 104 CFU/g or CFU/mL (10 cells/response) of E. faecalis and decrease cell load samples, after a Three h enrichment in NIOT-E. faecalis enrichment medium at 37 °C, a sensitivity degree of 10 CFU/g or CFU/mL was achieved.
GPR119 Polyclonal Antibody
40973-50ul
SAB
50ul
EUR 224.4
GPR119 Polyclonal Antibody
ABP53820-003ml
Abbkine
0.03ml
EUR 189.6
Description: A polyclonal antibody for detection of GPR119 from Human. This GPR119 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human GPR119 at AA rangle: 160-240
GPR119 Polyclonal Antibody
ABP53820-01ml
Abbkine
0.1ml
EUR 346.8
Description: A polyclonal antibody for detection of GPR119 from Human. This GPR119 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human GPR119 at AA rangle: 160-240
GPR119 Polyclonal Antibody
ABP53820-02ml
Abbkine
0.2ml
EUR 496.8
Description: A polyclonal antibody for detection of GPR119 from Human. This GPR119 antibody is for WB, IF, ELISA. It is affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in rabbit by using as an immunogen synthesized peptide derived from the Internal region of human GPR119 at AA rangle: 160-240
GPR119 Polyclonal Antibody
ES4819-100ul
ELK Biotech
100ul
EUR 334.8
Description: A Rabbit Polyclonal antibody against GPR119 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA
GPR119 Polyclonal Antibody
ES4819-50ul
ELK Biotech
50ul
EUR 248.4
Description: A Rabbit Polyclonal antibody against GPR119 from Human. This antibody is tested and validated for WB, ELISA, IF, WB, ELISA
GPR119 Polyclonal Conjugated Antibody
C40973
SAB
100ul
EUR 476.4
Polyclonal GPR119 Antibody (aa186-235)
APR16477G
Leading Biology
0.05ml
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR119 (aa186-235). This antibody is tested and proven to work in the following applications:
Polyclonal GPR119 Antibody (Cytoplasmic Domain)
APR16478G
Leading Biology
0.05mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR119 (Cytoplasmic Domain). This antibody is tested and proven to work in the following applications:
Polyclonal GPR119 Antibody (Cytoplasmic Domain)
APR16479G
Leading Biology
0.05mg
EUR 580.8
Description: A polyclonal antibody raised in Rabbit that recognizes and binds to Human GPR119 (Cytoplasmic Domain). This antibody is tested and proven to work in the following applications:
G-Protein Coupled Receptor 119 (GPR119) Antibody
abx215630-100ug
Abbexa
100 ug
EUR 526.8
G-Protein Coupled Receptor 119 (GPR119) Antibody
20-abx121407
Abbexa
EUR 360.00
EUR 526.80
EUR 226.80
G-Protein Coupled Receptor 119 (GPR119) Antibody
abx432763-200ul
Abbexa
200 ul
EUR 460.8
G Protein-Coupled Receptor 119 (GPR119) Antibody
20-abx324143
Abbexa
GPR119 siRNA
20-abx918416
Abbexa
GPR119 siRNA
20-abx918417
Abbexa
GPR119 siRNA
20-abx902267
Abbexa
GPR119 Peptide
45-692P
ProSci
0.1 mg
EUR 405.6
Description: GPR119 Peptide
GPR119 Rabbit pAb
A18544-100ul
Abclonal
100 ul
EUR 369.6
GPR119 Rabbit pAb
A18544-200ul
Abclonal
200 ul
EUR 550.8
GPR119 Rabbit pAb
A18544-20ul
Abclonal
20 ul
EUR 219.6
GPR119 Rabbit pAb
A18544-50ul
Abclonal
50 ul
EUR 267.6
GPR119 cloning plasmid
CSB-CL840575HU-10ug
Cusabio
10ug
EUR 279.6
Description: A cloning plasmid for the GPR119 gene.
GPR119 Blocking Peptide
DF4892-BP
Affbiotech
1mg
EUR 234
GPR119 Blocking Peptide
20-abx162463
Abbexa
anti- GPR105 antibody
FNab03588
FN Test
100µg
EUR 658.5
Description: Antibody raised against GPR105
anti- GPR107 antibody
FNab03589
FN Test
100µg
EUR 702
Description: Antibody raised against GPR107
anti- GPR108 antibody
FNab03590
FN Test
100µg
EUR 658.5
Description: Antibody raised against GPR108
anti- GPR116 antibody
FNab03591
FN Test
100µg
EUR 658.5
Description: Antibody raised against GPR116
anti- GPR132 antibody
FNab03593
FN Test
100µg
EUR 606.3
Description: Antibody raised against GPR132
anti- GPR137 antibody
FNab03594
FN Test
100µg
EUR 606.3
Description: Antibody raised against GPR137
anti- GPR142 antibody
FNab03597
FN Test
100µg
EUR 702
Description: Antibody raised against GPR142
anti- GPR161 antibody
FNab03598
FN Test
100µg
EUR 606.3
Description: Antibody raised against GPR161
anti- GPR162 antibody
FNab03599
FN Test
100µg
EUR 702
Description: Antibody raised against GPR162
anti- GPR177 antibody
FNab03601
FN Test
100µg
EUR 658.5
Description: Antibody raised against GPR177
Rat GPR119 shRNA Plasmid
20-abx989158
Abbexa
Human GPR119 shRNA Plasmid
20-abx965265
Abbexa
Mouse GPR119 shRNA Plasmid
20-abx982252
Abbexa
anti- GPR137B antibody
FNab03595
FN Test
100µg
EUR 702
Description: Antibody raised against GPR137B
GPR119 Recombinant Protein (Rat)
RP203282
ABM
100 ug
Ask for price
anti- GPR17 antibody
FNab03600
FN Test
100µg
EUR 658.5
Description: Antibody raised against GPR17
anti- GPR22 antibody
FNab03603
FN Test
100µg
EUR 606.3
Description: Antibody raised against GPR22
anti- GPR35 antibody
FNab03605
FN Test
100µg
EUR 702
Description: Antibody raised against GPR35
anti- GPR50 antibody
FNab03607
FN Test
100µg
EUR 658.5
Description: Antibody raised against GPR50
anti- GPR50 antibody
FNab03608
FN Test
100µg
EUR 658.5
Description: Antibody raised against GPR50
anti- GPR81 antibody
FNab03610
FN Test
100µg
EUR 702
Description: Antibody raised against GPR81
anti- GPR83 antibody
FNab03611
FN Test
100µg
EUR 702
Description: Antibody raised against GPR83
GPR119 Recombinant Protein (Mouse)
RP139418
ABM
100 ug
Ask for price
GPR119 Recombinant Protein (Human)
RP039541
ABM
100 ug
Ask for price
anti- GPR183/EBI2 antibody
FNab03602
FN Test
100µg
EUR 658.5
Description: Antibody raised against GPR183/EBI2
Anti-OR51E1/Gpr164 Antibody
A30874
BosterBio
100ul
EUR 476.4
Description: Rabbit Polyclonal OR51E1/Gpr164 Antibody. Validated in IF, WB and tested in Human.
Gpr119 ORF Vector (Rat) (pORF)
ORF067762
ABM
1.0 ug DNA
EUR 607.2
anti- GPR3 antibody
FNab03604
FN Test
100µg
EUR 702
Description: Antibody raised against GPR3
GPR119 ORF Vector (Human) (pORF)
ORF013181
ABM
1.0 ug DNA
EUR 424.8
Gpr119 ORF Vector (Mouse) (pORF)
ORF046474
ABM
1.0 ug DNA
EUR 607.2
anti- GPR101-Specific antibody
FNab03587
FN Test
100µg
EUR 702
Description: Antibody raised against GPR101-Specific
anti- GPR123-Specific antibody
FNab03592
FN Test
100µg
EUR 702
Description: Antibody raised against GPR123-Specific
anti- GPR139-Specific antibody
FNab03596
FN Test
100µg
EUR 606.3
Description: Antibody raised against GPR139-Specific
Anti-FFAR3/Gpr41 Antibody
A02437
BosterBio
100ul
EUR 476.4
Description: Rabbit Polyclonal FFAR3/Gpr41 Antibody. Validated in IF and tested in Human, Mouse.
anti- GPR58/TAAR2 antibody
FNab03609
FN Test
100µg
EUR 658.5
Description: Antibody raised against GPR58/TAAR2
Anti-SUCNR1/Gpr91 Antibody
A08485
BosterBio
100ul
EUR 476.4
Description: Rabbit Polyclonal SUCNR1/Gpr91 Antibody. Validated in WB and tested in Human, Mouse, Rat.
anti- GPR37/Pael-R antibody
FNab03606
FN Test
100µg
EUR 606.3
Description: Antibody raised against GPR37/Pael-R
Anti-GPCR GPR40/FFAR1 Antibody
A01349-2
BosterBio
100ug/vial
EUR 352.8
Gpr119 sgRNA CRISPR Lentivector set (Rat)
K7204201
ABM
3 x 1.0 ug
EUR 406.8
GPR119 sgRNA CRISPR Lentivector set (Human)
K0895801
ABM
3 x 1.0 ug
EUR 406.8
Gpr119 sgRNA CRISPR Lentivector set (Mouse)
K3605701
ABM
3 x 1.0 ug
EUR 406.8
Anti-LGR4/Gpr48 Monoclonal Antibody
M02134
BosterBio
100uL
EUR 531.6
Description: Mouse Monoclonal LGR4/Gpr48 Antibody. Validated in IHC, WB and tested in Human.
Gpr119 3'UTR GFP Stable Cell Line
TU158968
ABM
1.0 ml
Ask for price
GPR119 3'UTR GFP Stable Cell Line
TU059210
ABM
1.0 ml
EUR 2799.6
Gpr119 3'UTR GFP Stable Cell Line
TU255325
ABM
1.0 ml
Ask for price
Polyclonal Goat Anti-TAS1R2 / GPR71 Antibody
AMM05957G
Leading Biology
0.1 mg
EUR 580.8
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human Goat Anti-TAS1R2 / GPR71 . This antibody is tested and proven to work in the following applications:
GPR119 Protein Vector (Rat) (pPM-C-HA)
PV271048
ABM
500 ng
EUR 723.6
GPR119 Protein Vector (Rat) (pPB-C-His)
PV271046
ABM
500 ng
EUR 723.6
GPR119 Protein Vector (Rat) (pPB-N-His)
PV271047
ABM
500 ng
EUR 723.6
GPR119 Protein Vector (Rat) (pPM-C-His)
PV271049
ABM
500 ng
EUR 723.6
GPR119 Protein Vector (Human) (pPM-C-HA)
PV052723
ABM
500 ng
EUR 577.2
GPR119 Protein Vector (Mouse) (pPM-C-HA)
PV185896
ABM
500 ng
EUR 723.6
Anti-GPR119 Antibody
A07391
BosterBio
100ul
EUR 476.4
Description: Rabbit Polyclonal Antibody for GPR119 Antibody (GPR119) detection.tested for WB in Human.
Anti-GPR119 antibody
STJ93327
St John's Laboratory
200 µl
EUR 236.4
Description: Rabbit polyclonal to GPR119.
GPR119 Protein Vector (Human) (pPB-C-His)
PV052721
ABM
500 ng
EUR 577.2
GPR119 Protein Vector (Human) (pPB-N-His)
PV052722
ABM
500 ng
EUR 577.2
GPR119 Protein Vector (Human) (pPM-C-His)
PV052724
ABM
500 ng
EUR 577.2
GPR119 Protein Vector (Mouse) (pPB-C-His)
PV185894
ABM
500 ng
EUR 723.6
GPR119 Protein Vector (Mouse) (pPB-N-His)
PV185895
ABM
500 ng
EUR 723.6
GPR119 Protein Vector (Mouse) (pPM-C-His)
PV185897
ABM
500 ng
EUR 723.6
GPR119 3'UTR Luciferase Stable Cell Line
TU009210
ABM
1.0 ml
EUR 2799.6
Gpr119 3'UTR Luciferase Stable Cell Line
TU108968
ABM
1.0 ml
Ask for price
Gpr119 3'UTR Luciferase Stable Cell Line
TU205325
ABM
1.0 ml
Ask for price
Gpr119 sgRNA CRISPR Lentivector (Rat) (Target 1)
K7204202
ABM
1.0 ug DNA
EUR 184.8
Gpr119 sgRNA CRISPR Lentivector (Rat) (Target 2)
K7204203
ABM
1.0 ug DNA
EUR 184.8
Gpr119 sgRNA CRISPR Lentivector (Rat) (Target 3)
K7204204
ABM
1.0 ug DNA
EUR 184.8
Anti Human GPR120 Monoclonal Antibody (Clone No. 2B6)
KG138
Sceti
each
EUR 856.8
Description: The Anti Human GPR120 Monoclonal Antibody (Clone No. 2B6) is available in Europe and for worldwide shipping via Gentaur.
GPR119 sgRNA CRISPR Lentivector (Human) (Target 1)
K0895802
ABM
1.0 ug DNA
EUR 184.8
GPR119 sgRNA CRISPR Lentivector (Human) (Target 2)
K0895803
ABM
1.0 ug DNA
EUR 184.8
GPR119 sgRNA CRISPR Lentivector (Human) (Target 3)
K0895804
ABM
1.0 ug DNA
EUR 184.8
Gpr119 sgRNA CRISPR Lentivector (Mouse) (Target 1)
K3605702
ABM
1.0 ug DNA
EUR 184.8
Gpr119 sgRNA CRISPR Lentivector (Mouse) (Target 2)
K3605703
ABM
1.0 ug DNA
EUR 184.8
Gpr119 sgRNA CRISPR Lentivector (Mouse) (Target 3)
K3605704
ABM
1.0 ug DNA
EUR 184.8
Anti-LGR5/GPR49 Rabbit Monoclonal Antibody
M00239
BosterBio
100ug/vial
EUR 476.4
Description: Rabbit Monoclonal LGR5/GPR49 Antibody. Validated in Flow Cytometry, IP, WB and tested in Human, Mouse, Rat.
Anti-GPCR GPR7/NPBWR1 Antibody
A08247-1
BosterBio
100ug/vial
EUR 352.8
Anti Mouse GPR40 Monoclonal Antibody (Clone No. G16)
KG116
Sceti
each
EUR 919.2
Description: The Anti Mouse GPR40 Monoclonal Antibody (Clone No. G16) is available in Europe and for worldwide shipping via Gentaur.
GPR119 Lentiviral Vector (Rat) (CMV) (pLenti-GIII-CMV)
LV629167
ABM
1.0 ug DNA
EUR 818.4
GPR119 Lentiviral Vector (Rat) (UbC) (pLenti-GIII-UbC)
LV629171
ABM
1.0 ug DNA
EUR 818.4
GPR119 Lentiviral Vector (Rat) (EF1a) (pLenti-GIII-EF1a)
LV629172
ABM
1.0 ug DNA
EUR 818.4
Rabbit Anti Human Gpr15 (N-Terminal) Polyclonal Antibody
CPBT-65692RH
Creative Diagnostics
0.1 mg
EUR 696
GPR105 (GPR105) Antibody
abx233588-100ug
Abbexa
100 ug
EUR 610.8
GPR105 (GPR105) Antibody
20-abx217588
Abbexa
GPR105 (GPR105) Antibody
20-abx008376
Abbexa
EUR 360.00
EUR 526.80
EUR 226.80
GPR80 / GPR99 Antibody
20-abx147443
Abbexa
GPR87 / GPR95 Antibody
20-abx147553
Abbexa
GPR92 / GPR93 Antibody
20-abx147570
Abbexa
GPR86 / GPR94 Antibody
20-abx147612
Abbexa
GPR80/GPR99 Antibody
44941-100ul
SAB
100ul
EUR 302.4
GPR80/GPR99 Antibody
44941-50ul
SAB
50ul
EUR 224.4
GPR83/GPR72 Antibody
44944-100ul
SAB
100ul
EUR 302.4
GPR83/GPR72 Antibody
44944-50ul
SAB
50ul
EUR 224.4
GPR86/GPR94 Antibody
44945-100ul
SAB
100ul
EUR 302.4
GPR86/GPR94 Antibody
44945-50ul
SAB
50ul
EUR 224.4
GPR87/GPR95 Antibody
44946-100ul
SAB
100ul
EUR 302.4
GPR87/GPR95 Antibody
44946-50ul
SAB
50ul
EUR 224.4
GPR92/GPR93 Antibody
44948-100ul
SAB
100ul
EUR 302.4
GPR92/GPR93 Antibody
44948-50ul
SAB
50ul
EUR 224.4
GPR83/GPR72 Antibody
DF4977
Affbiotech
200ul
EUR 420
GPR87/GPR95 Antibody
DF4978
Affbiotech
200ul
EUR 420
GPR92/GPR93 Antibody
DF4979
Affbiotech
200ul
EUR 420
GPR86/GPR94 Antibody
DF5139
Affbiotech
200ul
EUR 420
GPR80/GPR99 Antibody
DF2765
Affbiotech
200ul
EUR 420
GPR83/GPR72 Antibody
DF2768
Affbiotech
200ul
EUR 420
GPR86/GPR94 Antibody
DF2770
Affbiotech
200ul
EUR 420
GPR87/GPR95 Antibody
DF2771
Affbiotech
200ul
EUR 420
GPR92/GPR93 Antibody
DF2774
Affbiotech
200ul
EUR 420
GPR109 Antibody
abx217169-100ug
Abbexa
100 ug
EUR 526.8
GPR182 antibody
20R-AR029
Fitzgerald
50 ug
EUR 787.2
Description: Rabbit polyclonal GPR182 antibody
GPR182 antibody
20R-AR030
Fitzgerald
50 ug
EUR 787.2
Description: Rabbit polyclonal GPR182 antibody
GPR182 antibody
20R-AR031
Fitzgerald
50 ug
EUR 787.2
Description: Rabbit polyclonal GPR182 antibody
GPR182 antibody
20R-AR032
Fitzgerald
50 ug
EUR 787.2
Description: Rabbit polyclonal GPR182 antibody
GPR182 antibody
20R-AR033
Fitzgerald
50 ug
EUR 787.2
Description: Rabbit polyclonal GPR182 antibody
GPR171 antibody
20R-GR028
Fitzgerald
50 ug
EUR 787.2
Description: Rabbit polyclonal GPR171 antibody
GPR175 antibody
20R-GR031
Fitzgerald
50 ug
EUR 787.2
Description: Rabbit polyclonal GPR175 antibody
GPR124 antibody
20R-GR039
Fitzgerald
50 ug
EUR 787.2
Description: Rabbit polyclonal GPR124 antibody
GPR115 antibody
20R-GR040
Fitzgerald
50 ug
EUR 787.2
Description: Rabbit polyclonal GPR115 antibody
GPR153 antibody
20R-GR042
Fitzgerald
50 ug
EUR 787.2
Description: Rabbit polyclonal GPR153 antibody
GPR132 antibody
20R-GR045
Fitzgerald
50 ug
EUR 787.2
Description: Rabbit polyclonal GPR132 antibody
GPR174 antibody
20R-GR051
Fitzgerald
50 ug
EUR 787.2
Description: Rabbit polyclonal GPR174 antibody
GPR152 antibody
20R-GR058
Fitzgerald
50 ug
EUR 787.2
Description: Rabbit polyclonal GPR152 antibody
GPR151 antibody
20R-GR062
Fitzgerald
50 ug
EUR 787.2
Description: Rabbit polyclonal GPR151 antibody
GPR149 antibody
20R-GR065
Fitzgerald
50 ug
EUR 787.2
Description: Rabbit polyclonal GPR149 antibody
GPR149 antibody
20R-GR066
Fitzgerald
50 ug
EUR 787.2
Description: Rabbit polyclonal GPR149 antibody
GPR160 antibody
20R-GR067
Fitzgerald
50 ug
EUR 787.2
Description: Rabbit polyclonal GPR160 antibody
GPR114 antibody
23178-100ul
SAB
100ul
EUR 468
GPR182 Antibody
37423-100ul
SAB
100ul
EUR 302.4
GPR124 Antibody
37603-100ul
SAB
100ul
EUR 302.4
GPR171 Antibody
37604-100ul
SAB
100ul
EUR 302.4
GPR143 Antibody
39269-100ul
SAB
100ul
EUR 468
GPR139 Antibody
44331-100ul
SAB
100ul
EUR 302.4
GPR139 Antibody
44331-50ul
SAB
50ul
EUR 224.4
GPR112 Antibody
44576-100ul
SAB
100ul
EUR 302.4
GPR112 Antibody
44576-50ul
SAB
50ul
EUR 224.4
GPR125 Antibody
44577-100ul
SAB
100ul
EUR 302.4
GPR125 Antibody
44577-50ul
SAB
50ul
EUR 224.4
GPR179 Antibody
44578-100ul
SAB
100ul
EUR 302.4
GPR179 Antibody
44578-50ul
SAB
50ul
EUR 224.4
GPR128 Antibody
44911-100ul
SAB
100ul
EUR 302.4
GPR128 Antibody
44911-50ul
SAB
50ul
EUR 224.4
GPR133 Antibody
44912-100ul
SAB
100ul
EUR 302.4
GPR133 Antibody
44912-50ul
SAB
50ul
EUR 224.4
GPR136 Antibody
44913-100ul
SAB
100ul
EUR 302.4
GPR136 Antibody
44913-50ul
SAB
50ul
EUR 224.4
GPR148 Antibody
44914-100ul
SAB
100ul
EUR 302.4
GPR148 Antibody
44914-50ul
SAB
50ul
EUR 224.4
GPR110 Antibody
44961-100ul
SAB
100ul
EUR 302.4
GPR110 Antibody
44961-50ul
SAB
50ul
EUR 224.4
GPR141 Antibody
44962-100ul
SAB
100ul
EUR 302.4
GPR141 Antibody
44962-50ul
SAB
50ul
EUR 224.4
GPR161 Antibody
44963-100ul
SAB
100ul
EUR 302.4
GPR161 Antibody
44963-50ul
SAB
50ul
EUR 224.4
GPR162 Antibody
44964-100ul
SAB
100ul
EUR 302.4