Because the emergence of novel Coronavirus (SARS-CoV-2) an infection in Wuhan, China in December 2019, it has now unfold to over 205 international locations. The ever-growing checklist of worldwide unfold corona virus-19 illness (COVID-19) sufferers has demonstrated the excessive transmission charge among the many human inhabitants. At the moment, there aren’t any FDA authorised medication or vaccines to stop and deal with the an infection of the SARS-CoV-2. Contemplating the present state of affairs, there’s an pressing unmet medical must determine novel and efficient approaches for the prevention and remedy of COVID-19 by re-evaluating the information of conventional medicines and repurposing of medicine.
Right here, we used molecular docking and molecular dynamics simulation strategy to discover the useful roles of phytochemicals and lively pharmacological brokers current within the Indian herbs that are broadly used within the preparation of Ayurvedic medicines within the type of Kadha to regulate varied respiratory issues reminiscent of cough, chilly and flu. Our research has recognized an array of phytochemicals current in these herbs which have important docking scores and potential to inhibit completely different phases of SARS-CoV-2 an infection in addition to different Coronavirus goal proteins.
The phytochemicals current in these herbs possess important anti-inflammatory property. Other than this, primarily based on their pharmaceutical traits, we’ve got additionally carried out in-silico drug-likeness and predicted pharmacokinetics of the chosen phytochemicals discovered within the Kadha. Total our research offers scientific justification by way of binding of lively substances current in numerous crops utilized in Kadha preparation with viral proteins and goal proteins for prevention and remedy of the COVID-19. Communicated by Ramaswamy H. Sarma.
Biomarker Evaluation Primarily based Chemoprofiling of Polyherbal Ayurvedic Formulation Containing Vitis vinifera L. by Validated UPLC-MS/MS Technique
Background: Drakshasava is among the business Ayurvedic medication from India, ready from grapes and spices. It’s believed to deal with well being imbalances and claimed to be useful for weak point bleeding issues and varied inflammatory ailments. It reported pharmacological actions reminiscent of diuretic, cardioprotective and antimicrobial. Being a polyherbal combination, it faces challenges in its standardization and high quality management. Goal The intention of the current research is to develop a validated UPLC-MS/MS methodology for simultaneous quantification of ten polyphenolic biomarkers in Drakshasava. It explores the impact of Vitis vinifera L. and extra herbs on fermentation with respect to bioactive compounds via successive addition methodology.
Technique: The MS strategies have been optimized in multiple-reaction monitoring (MRM) mode with ESI whereas chromatographic separation was achieved on an Acquity UPLC BEH C18 column utilizing each isocratic and gradient elution in water and acetonitrile containing 0.1% formic acid.
Outcomes: The developed methodology was validated as per ICH-Q2B tips and located to be throughout the assay variability limits. Gallic acid was discovered to be essentially the most considerable marker in all of the samples adopted by resveratrol. The content material of all of the markers has discovered to be elevated considerably submit fermentation, in comparison with decoction besides kaempferol. The successive addition of prashpeka drvya (minor herbs) within the formulation, present variability at completely different phases, with respect to the chosen markers and doesn’t infer main adjustments within the chemical profiling of ultimate product.
Conclusion: The developed methodology was discovered fast, correct, dependable and extremely delicate for the simultaneous quantification of chosen biomarkers in Drakshasava. The analysis is the primary chemometric report in standardization of Drakshasava by validated UPLC-MS/MS methodology. It might show to be useful gizmo for growth of recent phyto-pharmaceutical drug and additional high quality management of different poly natural formulations.
Evaluation of traditional ayurvedic Kadha for prevention and management of the novel Coronavirus (SARS-CoV-2) using in silico approach
Immunoprotective potential of Ayurvedic herb Kalmegh (Andrographis paniculata) in opposition to respiratory viral infections – LC-MS/MS and community pharmacology evaluation
Introduction: Immunity boosting has emerged as a worldwide technique to combat the SARS-CoV-2 pandemic scenario. In India, AYUSH methods of medication have been promoted as an immune-protection technique. Andrographis paniculata (Burm. F) Nees (AP) talked about in Ayurveda has been broadly used for treating sore throat, flu, and higher respiratory tract infections which can present potential novel therapeutic approaches, completely focusing on SARS-CoV-2 and its pathways.
Targets: The current work makes use of liquid chromatography-tandem mass spectrometry (LC-MS/MS) metabolomics and mixture synergy evaluation primarily based on community pharmacology to mine multimode proof to grasp the potential mechanism of motion, ailments affiliation, protein-protein interplay and main pathways concerned therein.
Materials and strategies: Metabolite profiling was carried out by Agilent QTOF LC-MS/MS system. Community pharmacology evaluation was carried out through the use of useful annotation evaluation primarily based on databases like Binding DB, STRING, DAVID and KEGG for additional knowledge mining. Additional mixture synergy was evaluated utilizing “neighbourhood strategy” and networks have been constructed via Cytoscape 3.2.1.
Description: A sandwich ELISA for quantitative measurement of Mouse Podoplanin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse Podoplanin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: A sandwich ELISA for quantitative measurement of Mouse Podoplanin in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.
Description: Podoplanin, also known as glycoprotein 38 (gp38), PA2.26 antigen, T1alpha (T1A), and aggrus, is a 38 kDa type I transmembrane sialoglycoprotein and member of the podoplanin family. Podoplanin is synthesized as a 172 amino acid (aa) precursor with a 22 aa signal sequence, a 119 aa extracellular domain (ECD), a 21 aa transmembrane region, and a short, 10 aa cytoplasmic tail. The ECD contains abundant Ser/Thr residues as potential sites for Oglycosylation, and the cytoplasmic region contains putative sites for kinase C and cAMP phosphorylation. Mouse Podoplanin shares 77% and 46% aa sequence identity with rat and human Podoplanin, respectively. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and on numerous tumors including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for Ctype lectin like receptor 2 (CLEC2). Their association is dependent on sialic acid on Oglycans of Podoplanin. Through its association with CLEC2, Podoplanin induces platelet aggregation and tumor metastasis. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.
Description: Podoplanin, also known as glycoprotein 38 (gp38), PA2.26 antigen, T1alpha (T1A), and aggrus, is a 38 kDa type I transmembrane sialoglycoprotein and member of the podoplanin family. Podoplanin is synthesized as a 172 amino acid (aa) precursor with a 22 aa signal sequence, a 119 aa extracellular domain (ECD), a 21 aa transmembrane region, and a short, 10 aa cytoplasmic tail. The ECD contains abundant Ser/Thr residues as potential sites for Oglycosylation, and the cytoplasmic region contains putative sites for kinase C and cAMP phosphorylation. Mouse Podoplanin shares 77% and 46% aa sequence identity with rat and human Podoplanin, respectively. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and on numerous tumors including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for Ctype lectin like receptor 2 (CLEC2). Their association is dependent on sialic acid on Oglycans of Podoplanin. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.
Description: Podoplanin, also known as glycoprotein 38 (gp38), PA2.26 antigen, T1alpha (T1A), and aggrus, is a 38 kDa type I transmembrane sialoglycoprotein and member of the podoplanin family. Podoplanin is synthesized as a 172 amino acid (aa) precursor with a 22 aa signal sequence, a 119 aa extracellular domain (ECD), a 21 aa transmembrane region, and a short, 10 aa cytoplasmic tail. The ECD contains abundant Ser/Thr residues as potential sites for Oglycosylation, and the cytoplasmic region contains putative sites for kinase C and cAMP phosphorylation. Mouse Podoplanin shares 77% and 46% aa sequence identity with rat and human Podoplanin, respectively. Podoplanin is expressed on glomerular epithelial cells (podocytes), type I lung alveolar cells, lymphatic endothelial cells, and on numerous tumors including colorectal tumors, squamous cell carcinomas, testicular seminoma, and brain tumors. One study shows high expression of Podoplanin mRNA in placenta, lung, skeletal muscle, and heart, and weaker levels in brain, kidney, and liver. Podoplanin is the ligand for Ctype lectin like receptor 2 (CLEC2). Their association is dependent on sialic acid on Oglycans of Podoplanin. Podoplanin is also necessary for lymphatic vessel formation, normal lung cell proliferation and alveolus formation at birth.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Podoplanin (PDPN) in samples from tissue homogenates, cell lysates or other biological fluids.
Description: A sandwich quantitative ELISA assay kit for detection of Mouse Podoplanin (PDPN) in samples from tissue homogenates, cell lysates or other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Podoplanin (PDPN) in tissue homogenates, cell lysates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Podoplanin (PDPN) in tissue homogenates, cell lysates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Podoplanin (PDPN) in tissue homogenates, cell lysates and other biological fluids.
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Mouse Podoplanin (PDPN) in tissue homogenates, cell lysates and other biological fluids.
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Mouse Podoplanin (PDPN) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.
Outcomes: The molecules from kalmegh offers immune-protection and anti-viral response through involving completely different pathways, like toll-like receptor pathway, PI3/AKT pathway and MAP kinase pathways in opposition to COVID-19 an infection. The KEGG evaluation confirmed that in a overwhelming majority of essentially the most enriched pathways, AP have been related to viral infections and higher respiratory tract infections.
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